AgriPheno提供农作物重要性状功能基因定位服务，结合芯片和高通量测序开发材料间的SNP位点，并结合高通量表型测试，提供基因型-表型-育种的整套实验流程设计与服务。

超高通量基因分型-分子标记检测平台-Nexar

Nexar系统是快速、自动化的内联仪器，包括Nexar^®模块化内联液处理与分析系统、Soellex^®高通量PCR水浴热循环系统和Araya^®内联荧光检测系统，可支持样本和阵列的高通量处理。Nexar利用创新的ArrayTape^TM（阵列卷带）系列耗材，能够在高精确度和准确性下运行。同时配备KASP Array Tape Master mix进行竞争性等位基因特异性PCR，可在广泛的基因组DNA样品中，对SNPs和特定位点上的InDels进行精准的双等位基因分型。

Nexar系统模块化内联平台与Array Tape的设计实现了基于微孔板技术的多功能性，并且几乎消除了手动操作和复杂的仪器操作。这种灵活的微孔板替代品可促进整个实验室的内联和整合自动化，并有助于终点PCR，qPCR和终点等温DNA扩增的应用。每个96孔，384孔和768孔阵列都标有独特的条形码标签，确保在处理过程中和加工后准确识别各个样品。此外，还可提供定制化的阵列，包括RNAse，DNAse和无热原选项。

Nexar 系统

Ÿ   Nexar^®

Nexar作为超高通量解决方案，是一套用于Array Tape（阵列卷带）样品和试剂处理的内联液体处理系统。该仪器提供分装、密封和源板存储，以及可选进程，如孵育和脱水。这一灵活的模块配置可以处理各种应用进程。

Ÿ   Soellex^®

Soellex为三室水浴热循环系统，能够在一轮运行中同时热循环多达三个Array Tape（阵列卷带）线轴（230,400个反应孔）或152个微孔板（384孔）。

Ÿ   Araya^®

Araya是内联荧光检测系统，专为Array Tape（阵列卷带）的自动扫描而设计。该系统可作为独立仪器或Nexar的内联模块使用。

Ÿ   Intellics^®

创新的配套软件提供集中数据管理、仪器监控、智能运行优化、Protocol生成和简化数据分析。

应用领域

Ÿ   粮食作物：水稻、小麦、玉米、土豆等

Ÿ   模式植物：拟南芥、烟草、二穗短柄草等

Ÿ   蔬菜作物：生菜、番茄、甜菜、黄瓜、甘蓝、菠菜等

Ÿ   模式动物：人、小白鼠、斑马鱼等

Ÿ   常见牲畜：牛、猪、山羊等

应用案例

图1 典型的基因分型富集图

注：每个数据点代表一个独立的DNA样本的荧光信号，相同基因型的样本会发出相似水平的荧光，因此富集在一起。

 服务项目

•     目标基因/性状标记开发 •     全基因组标记开发 •     遗传图谱的构建 •     图位克隆 •     背景筛查 •     蔬菜等纯度检测 •     植物身份鉴定/分群

服务特点

•     适用于超高通量自动化检测，液体处理、孵育与检测等流程一体化

•     反应体系微型化，显著降低单个数据点的成本

•     兼容粗提的DNA样本，并可灵活选用化学试剂

•     专业化生物信息平台及团队

•     大规模数据存储及数据处理服务器

客户提供

•     基因或指定区间信息

•     符合要求的供体/受体材料的DNA、叶片或种子等植物组织

服务周期

•     根据特定序列中变异位点设计标记：10-25个工作日

•     根据特定供体与特定回交亲本开发：10-20个工作日

•     基因初步定位：30～40个工作日

•     基因精细定位：30～40个工作日

•     已克隆基因序列开发: 15-30个工作日

•     全基因组标记开发：4-6个月

•     未克隆已定位基因标记开发：根据性状复杂程度确定

视频链接：https://v.youku.com/v_show/id_XNDAzMDE5NjU2OA==.html?spm=a2h3j.8428770.3416059.1

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2014

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2013

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2012

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